- $31
- sciencell
- 美国
- 09241
- 2025年12月17日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
NA
- 保质期：
NA
- 英文名：
DMEM with High-Glucose, L-Glutamine, Sodium Pyruvate and HEPES
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
09241
- 规格：
1 kit
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文献和实验美国密歇根大学的3T3-L1细胞分化的PROTOCOL(非常详细)3T3-L1 Differentiation ProtocolMATERIALSDulbecco's Modified Eagles Medium (DMEM; GibcoBRL-Cat# 11965-084: high glucose, with L-glutamine, with pyroxidine HCl, without sodium pyruvate)Calf Serum (GibcoBRL-Cat#16170
is dissolved. (Note: Do not heat to quicken the process.) 5) Once all of the media is dissolved, add 1.176-g NaHCO3 (Sodium Bicarbonate) and stir until dissolved. 6) Add 5 mLs Sodium Pyruvate/Liter of media 7) Next pH the solution to 7.15 with 1N NaOH
Sodium Pyruvate (keep this sterile) 6) pH to 7.2 with 1N NaOH 7) bring up to 900 mls with ddH2 O-test pH 8) 450 mls media per sterile filter container 9) re-pH small sample poured into Falcon tube to assure filtering did not change pH of media
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