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文献和实验(1.6x-2x) might "stick" the newly synthesized strands better to the template, allowing the polymerase to finish its task. PCR reactions in which only KCl or Tris-HCl concentrations were varied, showed that the described effect is due to the salt
Shorty DNA Prep (per Adam 08/12/04)【University of Chicago】
sit at room temperature overnight. Shorty Buffer (500mL) Glycogen (10mg/L) 1M Tris-HCl, pH 9.0 2M LiCl* 0.5M EDTA 10% SDS H2 0 500µl 100ml 100ml 25ml
posttranslational modifications of histones, and the cumbersome use of reverse-phase high-performance liquid chromatography. The three methodologies are shorter than the conventionally used protocols. The salt-urea method exploits the stability of the cell nucleus
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