即用型Bradford蛋白定量试剂盒(兼容去垢剂)
Ready-to-use Bradford Protein Assay Kit (Detergent Compatible)
本产品冰袋运输; 即用型BSA标准品 -20℃保存,其它组分4℃保存,保质期12个月。
货号规格
goodZJ104 800次(微孔)
产品内容
组分名称 |
体积 |
Bradford定量试剂 |
250 mL |
即用型BSA标准品①( 0 μg/mL ) |
1 mL |
即用型BSA标准品②(100 μg/mL) |
1 mL |
即用型BSA标准品③(150 μg/mL) |
1 mL |
即用型BSA标准品④(200 μg/mL) |
1 mL |
即用型BSA标准品⑤(300 μg/mL) |
1 mL |
即用型BSA标准品⑥(400 μg/mL) |
1 mL |
即用型BSA标准品⑦(500 μg/mL) |
1 mL |
产品特点
g
d方便快捷——提供即用型标准品,省去繁琐的稀释步骤;
g
d兼容性好——与金属离子、还原剂、螯合剂及去污剂兼容性较好。
产品简介
goodBradford 蛋白浓度测定方法,是常用的经典蛋白浓度检测方法。其原理是考马斯亮蓝 G-250 与蛋白质的碱性和芳香族氨基酸结合后,产生蓝色化合物,化合物颜色深浅程度与蛋白浓度在一定范围内有较好的线性关系,因此可通过检测 595 nm 的最大光吸收值来计算蛋白质浓度。
good本产品对传统的 Bradford 方法进行了改良,最佳检测范围为 100~500 μg/mL,在保留其检测速度快,兼容高浓度还原剂等优点的同时,还能耐受 1% SDS、1% NP-40、1% Triton X-100、1% Brij35 和 1% Tween 20 等各种常用去垢剂,从而可以与市面上大多数蛋白裂解液兼容。同时,本试剂盒配备有一系列梯度浓度的蛋白质标准品溶液 (BSA 溶液 ),即取即用,无需稀释,方便快捷。
使用说明
good◈以微孔酶标仪法为例:
gooddd1. 分别取 即用型 BSA 标准品①~⑦ 各 10 μL 加到 96 孔板中 ( BSA 标准品 使用前须充分溶解摇匀 );
gooddd2. 用 1×PBS 或 0.9% 生理盐水将样品适当稀释 ( 可以多作几个梯度,如 2 倍、4 倍、8 倍稀释 ),加 10 μL到 96 孔板的样品孔中;
gooddd3. 各孔加入 300 μL Bradford 定量试剂 ,充分混匀,盖上 96 孔板盖,室温孵育 3~5 min;
gooddd4. 用酶标仪测定每个样品及 BSA 标准品的 A595,注意要减去空白对照 ( 标准品① + Bradford 定量试剂 ) 的吸光度;
gooddd5. 以蛋白标准品的浓度为横坐标,吸光值为纵坐标,绘制标准曲线,得到标准曲线线性公式及 R2 值,计算样品的蛋白浓度。
gooddd5. 注意:计算样品蛋白浓度时,所测得的浓度值需乘以样品的稀释倍数。
注意事项
good1. 如通过分光光度计使用本产品测定蛋白浓度,需根据比色皿的最小检测体积,适当扩大反应体系,确保蛋白样品 ( 标准品 ) 与 Bradford 定量试剂 的用量体积比为 1:30 即可。使用分光光度计测定蛋白浓度时,每个试剂盒可以测定的样品数量可能会显著减少;
good2. 当去垢剂浓度大于 1% 时,部分去垢剂和去垢剂组合仍然适用于本产品,但可能会出现标准曲线斜率减小的现象,影响结果准确度;
good3. 试剂在低温条件或长期保存出现沉淀时,请上下翻转混匀溶解;
good4. 建议每次测定蛋白样品浓度时,都须绘制标准曲线,以获得准确数据;
good5. 为了您的安全和健康,请穿实验服并戴一次性手套操作;
good6. 本产品仅限科研使用。
相关论文
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good7. LIU, Heze, et al. Prenatal dexamethasone exposure induces nonalcoholic fatty liver disease in male rat offspring via the miR-122/YY1/ACE2-MAS1 pathway. Biochemical Pharmacology, 2021, 185: 114420.(IF 5.858)
good8. PIAO, Jinmei, et al. Effects of real-ambient PM2. 5 exposure on lung damage modulated by Nrf2. Frontiers in pharmacology, 2021, 12: 913.(IF 5.810)
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good10. Feng, F., Cheng, P., Xu, S., Li, N., Wang, H., Zhang, Y., & Wang, W. (2020). Tanshinone IIA attenuates silica-induced pulmonary fibrosis via Nrf2-mediated inhibition of EMT and TGF-β1/Smad signaling. Chemico-Biological Interactions, 109024.(IF 5.192)
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