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- 2026年04月17日
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- 规格：
800次(微孔)
即用型Bradford蛋白定量试剂盒(兼容去垢剂)
Ready-to-use Bradford Protein Assay Kit (Detergent Compatible)
本产品冰袋运输； 即用型BSA标准品 -20℃保存，其它组分4℃保存，保质期12个月。
货号规格
goodZJ104 800次(微孔)
产品内容
| 组分名称 | 体积 |
| Bradford定量试剂 | 250 mL |
| 即用型BSA标准品①( 0 μg/mL ) | 1 mL |
| 即用型BSA标准品②(100 μg/mL) | 1 mL |
| 即用型BSA标准品③(150 μg/mL) | 1 mL |
| 即用型BSA标准品④(200 μg/mL) | 1 mL |
| 即用型BSA标准品⑤(300 μg/mL) | 1 mL |
| 即用型BSA标准品⑥(400 μg/mL) | 1 mL |
| 即用型BSA标准品⑦(500 μg/mL) | 1 mL |
产品特点
g
d方便快捷——提供即用型标准品，省去繁琐的稀释步骤；
g
d兼容性好——与金属离子、还原剂、螯合剂及去污剂兼容性较好。
产品简介
goodBradford 蛋白浓度测定方法，是常用的经典蛋白浓度检测方法。其原理是考马斯亮蓝 G-250 与蛋白质的碱性和芳香族氨基酸结合后，产生蓝色化合物，化合物颜色深浅程度与蛋白浓度在一定范围内有较好的线性关系，因此可通过检测 595 nm 的最大光吸收值来计算蛋白质浓度。
good本产品对传统的 Bradford 方法进行了改良，最佳检测范围为 100~500 μg/mL，在保留其检测速度快，兼容高浓度还原剂等优点的同时，还能耐受 1% SDS、1% NP-40、1% Triton X-100、1% Brij35 和 1% Tween 20 等各种常用去垢剂，从而可以与市面上大多数蛋白裂解液兼容。同时，本试剂盒配备有一系列梯度浓度的蛋白质标准品溶液 (BSA 溶液 )，即取即用，无需稀释，方便快捷。
使用说明
good◈以微孔酶标仪法为例：
gooddd1. 分别取 即用型 BSA 标准品①~⑦ 各 10 μL 加到 96 孔板中 ( BSA 标准品 使用前须充分溶解摇匀 )；
gooddd3. 各孔加入 300 μL Bradford 定量试剂 ，充分混匀，盖上 96 孔板盖，室温孵育 3~5 min；
gooddd4. 用酶标仪测定每个样品及 BSA 标准品的 A595，注意要减去空白对照 ( 标准品① ＋ Bradford 定量试剂 ) 的吸光度；
gooddd5. 以蛋白标准品的浓度为横坐标，吸光值为纵坐标，绘制标准曲线，得到标准曲线线性公式及 R2 值，计算样品的蛋白浓度。
gooddd5. 注意：计算样品蛋白浓度时，所测得的浓度值需乘以样品的稀释倍数。
注意事项
good1. 如通过分光光度计使用本产品测定蛋白浓度，需根据比色皿的最小检测体积，适当扩大反应体系，确保蛋白样品 ( 标准品 ) 与 Bradford 定量试剂 的用量体积比为 1:30 即可。使用分光光度计测定蛋白浓度时，每个试剂盒可以测定的样品数量可能会显著减少；
good2. 当去垢剂浓度大于 1% 时，部分去垢剂和去垢剂组合仍然适用于本产品，但可能会出现标准曲线斜率减小的现象，影响结果准确度；
good3. 试剂在低温条件或长期保存出现沉淀时，请上下翻转混匀溶解；
good4. 建议每次测定蛋白样品浓度时，都须绘制标准曲线，以获得准确数据；
good5. 为了您的安全和健康，请穿实验服并戴一次性手套操作；
good6. 本产品仅限科研使用。
相关论文
good1. Cheng, B., Zhang, H., Hu, J., Peng, Y., Yang, J., Liao, X., ... & Lu, H. (2020). The immunotoxicity and neurobehavioral toxicity of zebrafish induced by famoxadone-cymoxanil. Chemosphere, 247, 125870. (IF 7.086)
good2. WANG, Feng, et al. Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis. Stem cell research & therapy, 2021, 12.1: 1-15.（IF 6.832）
good3. XIAO, Lili, et al. Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro. Frontiers in Cell and Developmental Biology, 2021, 9: 350.（IF 6.684）
good4. WANG, Feng, et al. Accelerated Bone Regeneration by Astragaloside IV through Stimulating the Coupling of Osteogenesis and Angiogenesis. International Journal of Biological Sciences, 2021, 17.7: 1821.（IF 6.58）
good5. JIANG, Qian, et al. 4-Phenylbutyric acid accelerates rehabilitation of barrier function in intestinal porcine epithelial cell (IPEC-J2) monolayer model. Animal Nutrition, 2021.（IF 6.383）
good6. LIU, Chang, et al. Value of Pyruvate Carboxylase in Thyroid Fine-Needle Aspiration Wash-Out Fluid for Predicting Papillary Thyroid Cancer Lymph Node Metastasis. Frontiers in oncology, 2021, 11: 1625.（IF 6.244）
good7. LIU, Heze, et al. Prenatal dexamethasone exposure induces nonalcoholic fatty liver disease in male rat offspring via the miR-122/YY1/ACE2-MAS1 pathway. Biochemical Pharmacology, 2021, 185: 114420.（IF 5.858）
good8. PIAO, Jinmei, et al. Effects of real-ambient PM2. 5 exposure on lung damage modulated by Nrf2. Frontiers in pharmacology, 2021, 12: 913.（IF 5.810）
good9. ZHANG, Junqian, et al. Andrographolide Induces Noxa-Dependent Apoptosis by Transactivating ATF4 in Human Lung Adenocarcinoma Cells. Frontiers in pharmacology, 2021, 12: 924.（IF 5.810）
good10. Feng, F., Cheng, P., Xu, S., Li, N., Wang, H., Zhang, Y., & Wang, W. (2020). Tanshinone IIA attenuates silica-induced pulmonary fibrosis via Nrf2-mediated inhibition of EMT and TGF-β1/Smad signaling. Chemico-Biological Interactions, 109024.（IF 5.192）
good11. SU, Juanjuan, et al. Identification and Biochemical Characterization of a Surfactant-Tolerant Chondroitinase VhChlABC from Vibrio hyugaensis LWW-1. Marine Drugs, 2021, 19.7: 399.（IF 5.118）
good12. SONG, Lin; PENG, Juan; GUO, Xuejun. Exosomal lncRNA TCONS_00064356 derived from injured alveolar epithelial type II cells affects the biological characteristics of mesenchymal stem cells. Life Sciences, 2021, 278: 119568.（IF 5.037）
good13. ZHOU, Yixi, et al. Chronic exposure to environmentally realistic levels of diuron impacts the behaviour of adult marine medaka (Oryzias melastigma). Aquatic Toxicology, 2021, 105917.（IF 4.964）
good14. Zhao, Y., Tao, M., Wang, R., Guo, Y., & Wang, M. (2020). Japonicone V, a sesquiterpene lactone derivative from the flowers of Inula japonica, inhibits hepatitis E virus replication by targeting virus-associated autophagy. Journal of Functional Foods, 65, 103755.（IF 4.451）
good15. He, S., Chen, M., Lin, X., Lv, Z., Liang, R., & Huang, L. (2020). Triptolide inhibits PDGF-induced proliferation of ASMCs through G0/G1 cell cycle arrest and suppression of the AKT/NF-κB/cyclinD1 signaling pathway. European Journal of Pharmacology, 867, 172811.（IF 4.432）
good16. Kang, L., Miao, J. X., Cao, L. H., Miao, Y. Y., Liu, H., Xiang, L., & Song, Y. G. (2020). Total glucosides of herbaceous peony (Paeonia lactiflora Pall.) flower attenuate adenine-and ethambutol-induced hyperuricaemia in rats. Journal of Ethnopharmacology, 113054.（IF 4.360）
good17. HE, Zhidong, et al. The role of MEG3 in the proliferation of palatal mesenchymal cells is related to the TGFβ/Smad pathway in TCDD inducing cleft palate. Toxicology and Applied Pharmacology, 2021, 419: 115517.（IF 4.219）
good18. Su, J., Yu, B., Zhang, C., Yi, P., Li, H., Xu, C., ... & Chen, J. (2020). Long noncoding RNA HOXC-AS3 indicates a poor prognosis and regulates tumorigenesis by binding to YBX1 in breast cancer. American journal of translational research, 12(10), 6335. （IF 4.060）
good19. LANG, Jiangli, et al. High glucose activates ERK1/2 to stabilize AP1 and increase MMP9 expression in diabetic foot ulcers. Experimental Cell Research, 2021, 403.1: 112550.（IF 3.905）
good20. Yang, Y., Yang, X., Lin, Y., Yang, G., & Li, L. (2020). LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/OXPHOS related proteins. Biochemical and Biophysical Research Communications. （IF 3.575）
good2. WANG, Feng, et al. Adrenomedullin 2 improves bone regeneration in type 1 diabetic rats by restoring imbalanced macrophage polarization and impaired osteogenesis. Stem cell research & therapy, 2021, 12.1: 1-15.（IF 6.832）
good3. XIAO, Lili, et al. Molecular Behavior of HMGB1 in the Cochlea Following Noise Exposure and in vitro. Frontiers in Cell and Developmental Biology, 2021, 9: 350.（IF 6.684）
good4. WANG, Feng, et al. Accelerated Bone Regeneration by Astragaloside IV through Stimulating the Coupling of Osteogenesis and Angiogenesis. International Journal of Biological Sciences, 2021, 17.7: 1821.（IF 6.58）
good5. JIANG, Qian, et al. 4-Phenylbutyric acid accelerates rehabilitation of barrier function in intestinal porcine epithelial cell (IPEC-J2) monolayer model. Animal Nutrition, 2021.（IF 6.383）
good6. LIU, Chang, et al. Value of Pyruvate Carboxylase in Thyroid Fine-Needle Aspiration Wash-Out Fluid for Predicting Papillary Thyroid Cancer Lymph Node Metastasis. Frontiers in oncology, 2021, 11: 1625.（IF 6.244）
good7. LIU, Heze, et al. Prenatal dexamethasone exposure induces nonalcoholic fatty liver disease in male rat offspring via the miR-122/YY1/ACE2-MAS1 pathway. Biochemical Pharmacology, 2021, 185: 114420.（IF 5.858）
good8. PIAO, Jinmei, et al. Effects of real-ambient PM2. 5 exposure on lung damage modulated by Nrf2. Frontiers in pharmacology, 2021, 12: 913.（IF 5.810）
good9. ZHANG, Junqian, et al. Andrographolide Induces Noxa-Dependent Apoptosis by Transactivating ATF4 in Human Lung Adenocarcinoma Cells. Frontiers in pharmacology, 2021, 12: 924.（IF 5.810）
good10. Feng, F., Cheng, P., Xu, S., Li, N., Wang, H., Zhang, Y., & Wang, W. (2020). Tanshinone IIA attenuates silica-induced pulmonary fibrosis via Nrf2-mediated inhibition of EMT and TGF-β1/Smad signaling. Chemico-Biological Interactions, 109024.（IF 5.192）
good11. SU, Juanjuan, et al. Identification and Biochemical Characterization of a Surfactant-Tolerant Chondroitinase VhChlABC from Vibrio hyugaensis LWW-1. Marine Drugs, 2021, 19.7: 399.（IF 5.118）
good12. SONG, Lin; PENG, Juan; GUO, Xuejun. Exosomal lncRNA TCONS_00064356 derived from injured alveolar epithelial type II cells affects the biological characteristics of mesenchymal stem cells. Life Sciences, 2021, 278: 119568.（IF 5.037）
good13. ZHOU, Yixi, et al. Chronic exposure to environmentally realistic levels of diuron impacts the behaviour of adult marine medaka (Oryzias melastigma). Aquatic Toxicology, 2021, 105917.（IF 4.964）
good14. Zhao, Y., Tao, M., Wang, R., Guo, Y., & Wang, M. (2020). Japonicone V, a sesquiterpene lactone derivative from the flowers of Inula japonica, inhibits hepatitis E virus replication by targeting virus-associated autophagy. Journal of Functional Foods, 65, 103755.（IF 4.451）
good15. He, S., Chen, M., Lin, X., Lv, Z., Liang, R., & Huang, L. (2020). Triptolide inhibits PDGF-induced proliferation of ASMCs through G0/G1 cell cycle arrest and suppression of the AKT/NF-κB/cyclinD1 signaling pathway. European Journal of Pharmacology, 867, 172811.（IF 4.432）
good16. Kang, L., Miao, J. X., Cao, L. H., Miao, Y. Y., Liu, H., Xiang, L., & Song, Y. G. (2020). Total glucosides of herbaceous peony (Paeonia lactiflora Pall.) flower attenuate adenine-and ethambutol-induced hyperuricaemia in rats. Journal of Ethnopharmacology, 113054.（IF 4.360）
good17. HE, Zhidong, et al. The role of MEG3 in the proliferation of palatal mesenchymal cells is related to the TGFβ/Smad pathway in TCDD inducing cleft palate. Toxicology and Applied Pharmacology, 2021, 419: 115517.（IF 4.219）
good18. Su, J., Yu, B., Zhang, C., Yi, P., Li, H., Xu, C., ... & Chen, J. (2020). Long noncoding RNA HOXC-AS3 indicates a poor prognosis and regulates tumorigenesis by binding to YBX1 in breast cancer. American journal of translational research, 12(10), 6335. （IF 4.060）
good19. LANG, Jiangli, et al. High glucose activates ERK1/2 to stabilize AP1 and increase MMP9 expression in diabetic foot ulcers. Experimental Cell Research, 2021, 403.1: 112550.（IF 3.905）
good20. Yang, Y., Yang, X., Lin, Y., Yang, G., & Li, L. (2020). LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/OXPHOS related proteins. Biochemical and Biophysical Research Communications. （IF 3.575）
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文献和实验相关实验
目前常用比色法测定样品蛋白的含量：Bradford法（考马斯亮蓝法）、Lowry法（Folin-酚试剂法）、 BCA法等。但各有优缺点，大家可以根据具体情况选取。按相应蛋白质定量试剂盒操作说明操作，测定样品浓度。收集完蛋白样品后，为确保每个蛋白样品的上样量一致，需要测定每个蛋白样品的蛋白浓度。根据所使用的裂解液的不同，需要采用适当的蛋白浓度测定方法。因为不同的蛋白浓度测定方法对于一些去垢剂和还原剂等的兼容性差别很大，大家可以根据具体情况选取。Bradford法（考马斯亮蓝法）:考马斯亮蓝G
形成紫色络合物，测定其在562nm处的吸收值，并与标准曲线对比，即可计算待测蛋白的浓度。 现对这两种方法进行比较： 一、实验材料： 细胞总蛋白提取试剂盒（AR0103） M231 BCA蛋白定量试剂盒（AR0146） Commassie改良增强型蛋白质定量试剂盒（AR0145） 二、操作步骤： Commassie法： 1.将BSA冻干标准品（10mg/支）用生理盐水或PBS稀释成2000ug/ml，1000 ug/ml,500
免疫印迹法（immunobiotting test，IBT）
，需要测定每个蛋白样品的蛋白浓度。根据所使用的裂解液的不同，需要采用适当的蛋白浓度测定方法。因为不同的蛋白浓度测定方法对于一些去垢剂和还原剂等的兼容性差别很大。更方便的方法是选用成套的裂解液和定量试剂盒测定蛋白质。如果浓度过高，可按比例稀释，读出蛋白浓度后，再折算回原样品浓度。 3. 电泳(Electrophoresis) 配制SDS-PAGE凝胶，在收集的蛋白样品中加入适量浓缩的SDS-PAGE蛋白上样缓冲液。例如2X或5X的SDS-PAGE蛋白上样缓冲液。使用5X的SDS
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ZJ104 即用型Bradford蛋白定量试剂盒(兼容去垢剂)
￥358
