Sample:
Lane 1: Recombinant Human VEGF165 Protein at 500ng
Primary: Mouse Anti-VEGF165 Monoclonal Antibody at 1/1000 dilution (Cat.bsm-41660M)
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 21kD
Observed band size: 25kD
- ￥2500 - 5600
- Bioss
- bsm-41660M
- 2025年10月24日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Cyclin B1 Rabbit pAb, Biotin conjugated(bs-0572R-Bio)-100ul
￥2980HLA-C Mouse mAb, BF350 conjugated(bsm-43093M-BF350)-100ul
￥2980
phospho-GATA6 (Tyr271) Rabbit pAb(bs-5375R)-50ul/100ul/200ul
￥1180PGAM5 Rabbit pAb, Biotin conjugated(bs-18228R-Bio)-100ul
￥2980
ILDR2 Rabbit pAb(bs-16601R)-50ul/100ul/200ul
￥1180
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
100ug/200ug
| 规格： | 100ug | 产品价格： | ￥2500.0 |
|---|---|---|---|
| 规格： | 200ug | 产品价格： | ￥5600.0 |
| 产品编号 | bsm-41660M |
| 英文名称 | Human VEGF165 Mouse mAb |
| 中文名称 | 人血管内皮生长因子单克隆抗体 |
| 英文别名 | VEGF165; Vascuoar endothelial growth factor A; VEGF A; vascular endothelial growth factor A isoform 2 precursor; Vegf; VEGFA; MGC70609; MVCD1; VEGF; VPF; VEGFA_HUMAN; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF; VEGF A Precursor. |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Mouse |
| 免疫原 | Recombinant human VEGF165 Protein |
| 亚型 | IgG1 |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Monoclonal |
| 理论分子量 | 18 kDa |
| 储存液 | 0.01M PBS (pH7.4). |
| 研究领域 | Cancer > Cancer Metabolism > Response to hypoxia Cancer > Growth factors > VEGF Cancer > Invasion/microenvironment > Angiogenesis > Angiogenic growth factors Cardiovascular > Angiogenesis > Growth Factors > VEGF > VEGF Developmental Biology > Organogenesis > Angiogenesis and vasculogenesis Metabolism > Pathways and Processes > Cofactors, Vitamins / minerals > Co-factors Metabolism > Pathways and Processes > Metabolism processes > Hypoxia Metabolism > Types of disease > Cancer Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus Signal Transduction > Growth Factors/Hormones > VEGF |
| 亚基 | Homodimer; disulfide-linked. Also found as heterodimer with PGF (By similarity). |
| 亚细胞定位 | Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. |
| 组织特异性 | soform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. |
| 相似性 | Belongs to the PDGF/VEGF growth factor family. |
| 功能 | Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF), is a signal protein produced by cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature). |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {ELISA} | {1:5000-10000} |
Measured by its binding ability in a indirect ELISA. Immobilized Human VEGF165, His Tag (Cat. bs-41613P) at 2 μg/mL (100 μL/well) can bind Anti-Human VEGF165 monoclonal antibody, the minimum detection concentration is 0.5ng/mL
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice
of transgenic mice carrying immunoglobulin (Ig) genes in germ line configuration. The engineered mouse genome can undergo productive rearrangement in the B cell population, with the generation of mouse B lymphocytes expressing human Ig (hIg) chains. To avoid
Dual- and Triple-Color Fluorospot
antibodies (mAbs) to cytokines: Mouse mAb to human IFN-γ; mAb 1-D1K for coating and FITC-labeled mAb 7-B6-1 for detection. Mouse mAb to human IL-2; mAbs IL2-I/249 for coating and biotinylated mAb IL2-II for detection. If a third cytokine is included
Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
with anti c-kit MAb and the lineage-negative cells from human bone marrow are incubated with anti-CD34 and anti-CD38 MAb. With a starting population of 2–4 � 10e8 unfractionated mouse bone-marrow cells, a typical isolation procedure yields approx 2 � 10e4 FR
技术资料暂无技术资料 索取技术资料
Human VEGF165 Mouse mAb(bsm-41660M)-100ug/200ug
￥2500 - 5600
