博奥森 bioss bs-1345R 足细胞特异蛋白抗体

产品编号bs-1345R

英文名称Rabbit Anti-PODXL  antibody

中文名称足细胞特异蛋白抗体

别    名Podocalyxin; PCLP; PCLP1; podocalyxin-like isoform 1 precursor; Podocalyxin like protein; PODXL_HUMAN; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1; PCX; PODXL. 

订购信息：

50ul  100ul  200ul

产品编号bs-1345R

英文名称Rabbit Anti-PODXL  antibody

中文名称足细胞特异蛋白抗体

别    名Podocalyxin; PCLP; PCLP1; podocalyxin-like isoform 1 precursor; Podocalyxin like protein; PODXL_HUMAN; GCTM-2 antigen; Gp200; Podocalyxin-like protein 1; PC; PCLP-1; PCX; PODXL. 

研究领域细胞生物 

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human,Mouse,Rat

产品应用WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

理论分子量59kDa

细胞定位细胞膜

性    状Liquid

浓    度1mg/ml

免 疫 原KLH conjugated synthetic peptide derived from human PODXL: 451-558/558

亚    型IgG

纯化方法affinity purified by Protein A

缓 冲 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

PubMedPubMed

产品介绍This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]

 

Function:

Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neihboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apcal actin-dependent microvilli. Involved in the formation of a preaical plasma membrane subdomain to set up inital epithelial polarization and the apial lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.

 

Subcellular Location:

Apial cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane. In single attached epithelial cells is restricted to a preapcal pole on the free plasma membrane whereas other apial and basolateral proteins are not yet polarized. Colocalizes with SLC9A3R2 at the apial plasma membrane during epithelial polarization. Colocalizes with SLC9A3R1 at the trans-Golgi network (transiently) and at the apial plasma membrane. Its association with the membrane raft is transient. Colocalizes with actin filaments, EZR and SLC9A3R1 in a punctate pattern at the apcal cell surface where microvilli form. Colocalizes with EZR and SLC9A3R2 at the aical cell membrane of glomerular epithelium cells (By similarity). Forms granular, punctuated pattern, forming patches, preferentially adopting a polar distribution, located on the migrating poles of the cell or forming clusters along the terminal ends of filipodia establishing contact with the endothelial cells. Colocalizes with the submembrane actin of lamellipodia, particularly associated with ruffles. Colocalizes with vinculin at protrusions of cells. Colocalizes with ITGB1. Colocalizes with PARD3, PRKCI, EXOC5, OCLN, RAB11A and RAB8A in apcal membrane initiation sites (AMIS) during the generation of apcal surface and luminogenesis.

 

Tissue Specificity:

Glomerular epithelium cell (podocyte).

 

Similarity:

Belongs to the podocalyxin family.

 

SWISS:

O00592

 

Gene ID:

5420