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Chicken Cholecystokinin (CCK)

ELISA Kit试剂盒,orb1736686,biorbyt
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  • ¥4290
  • biorbyt已认证
  • 15.63-1000 pg/mL
  • 4.71 pg/mL
  • orb1736686
  • 英国
  • 2026年01月21日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      999

    • 供应商

      biorbyt

    • 检测范围

      15.63-1000 pg/mL

    • 检测方法

      Competitive

    • 适应物种

      Gallus

    • 样本

      serum, plasma, cell lysates, cell culture supernates and other biological fluids

    • 灵敏度

      4.71 pg/mL

    • 规格

      48 T

    产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve.

    产品别名:CCK-PZ

    应用笔记:standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve

    实验时长:2.5h

    UniProt ID:Q9PU41

    Note:For research use only.

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    图标文献和实验
    相关实验
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      Ubiquitin Expression Plasmids   Materials Chicken albumin or ovalbumin, KLH, or other suitable hapten Ubiquitin (from bovine erythrocytes; Sigma) 0.1 M potassium phosphate buffer, pH 7.0 ( appendix 2A

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