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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
biorbyt
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Competitive
- 适应物种:
Gallus
- 样本:
serum, plasma, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
4.71 pg/mL
- 规格:
48 T
产品描述:This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve.
产品别名:CCK-PZ
应用笔记:standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:2.5h
UniProt ID:Q9PU41
Note:For research use only.
产品别名:CCK-PZ
应用笔记:standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Chicken CCK protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chicken CCK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chicken CCK in the samples is then determined by comparing the OD of the samples to the standard curve
实验时长:2.5h
UniProt ID:Q9PU41
Note:For research use only.
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文献和实验相关实验
Studies of the Ubiquitin Proteasome System
Ubiquitin Expression Plasmids Materials Chicken albumin or ovalbumin, KLH, or other suitable hapten Ubiquitin (from bovine erythrocytes; Sigma) 0.1 M potassium phosphate buffer, pH 7.0 ( appendix 2A
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Chicken Cholecystokinin (CCK) ELISA Kit试剂盒,orb1736686,biorbyt
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