APG8a/b(MAP1LC3A/B) Antibody 抗体,orb1938826,Biorbyt产品图

APG8a/b(MAP1LC3A/B) Antibody 抗

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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1938826
  • 英国
  • 2026年08月07日
  • IF, IHC-P, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      APG8a/b(MAP1LC3A/B) Antibody 抗体

    • 抗体英文名

      APG8a/b(MAP1LC3A/B) Antibody

    • 靶点

      MAP1LC3B (HGNC:13352)

    • 应用范围

      IF, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1938826

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Affinity Purified Rabbit Polyclonal Antibody (Pab)

    别名: Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, MAP1LC3B, MAP1ALC3

    应用稀释比例: IF - 1:100, WB - 1:1000, IHC-P - 1:50-100

    免疫原: This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b. Antigen Region: 100-125 aa.

    分子量: 14688 Da

    预测反应性: Bovine

    研究领域: Cell Biology

    UniProt ID: Q9GZQ8

    NCBI 参考序列: NP_073729.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    APG8a/b (MAP1LC3A/B) western blot analysis in mouse lung tissue lysates (35 ug/lane). This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).

    产品细节图片2
    Cleaved-APG8a/b antibody (MAP1LC3A/B) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.

    产品细节图片3
    Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) Antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

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    图标文献和实验
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    • Antibody Conjugation

      8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody

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