MAPE Antibody (C-term) 抗体,orb1929852,Biorbyt产品图

MAPE Antibody (C-term) 抗体,orb1

929852,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1929852
  • 英国
  • 2026年08月07日
  • FC, IHC-P, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      MAPE Antibody (C-term) 抗体

    • 抗体英文名:

      MAPE Antibody (C-term)

    • 靶点:

      PRAME {ECO:0000303|PubMed:9047241, ECO:0000312|HGNC:HGNC:9336}

    • 应用范围:

      FC, IHC-P, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb1929852

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, OIP-4, Preferentially expressed antigen of melanoma, PRAME, MAPE, OIP4

    应用稀释比例: WB - 1:2000, IHC-P - 1:50-100, FC - 1:25

    免疫原: This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 amino acids from the C-terminal region of human MAPE. Antigen Region: 476-502 aa.

    分子量: 57890 Da

    研究领域: Cell Biology

    UniProt ID: P78395

    NCBI 参考序列: NP_996836.1, NP_996838.1, NP_996839.1, NP_006106.1, NP_996837.1

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

    产品细节图片2
    MAPE Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MAPE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

    产品细节图片3
    All lanes: Anti-MAPE Antibody (C-term) at 1:2000 dilution. Lane 1: Hela whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: human testis lysate. Lane 5: human brain lysate. Lane 6: human kidney lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 58 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

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