STMN2 Antibody (Center) 抗体,orb1926555,Biorbyt产品图

STMN2 Antibody (Center) 抗体,orb

1926555,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1926555
  • 英国
  • 2026年08月07日
  • FC, IF, IHC, WB
  • Rabbit
  • Human, Mouse
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名:

      STMN2 Antibody (Center) 抗体

    • 抗体英文名:

      STMN2 Antibody (Center)

    • 靶点:

      STMN2

    • 应用范围:

      FC, IF, IHC, WB

    • 宿主:

      Rabbit

    • 适应物种:

      Human, Mouse

    • 保质期:

      12 months from date of receipt.

    • 抗原来源:

      详询

    • 目录编号:

      orb1926555

    • 级别:

      科研

    • 库存:

      99

    • 供应商:

      Biorbyt

    • 标记物:

      Unconjugated

    • 克隆性:

      Polyclonal

    • 保存条件:

      详询

    • 形态:

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型:

      Rabbit IgG

    • 免疫原:

      详询

    • 规格:

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Stathmin-2, Superior cervical ganglion-10 protein, Protein SCG10, STMN2, SCG10, SCGN10

    应用稀释比例: IF - 1:25, WB - 1:1000, IHC - 1:25, FC - 1:25

    免疫原: This STMN2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 82-116 amino acids from the Central region of human STMN2.

    分子量: 20828 Da

    UniProt ID: Q93045

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    All lanes: Anti-STMN2 Antibody (Center) at 1:1000 dilution. Lane 1: human brain lysates. Lane 2: mouse brain lysates. Lane 3: SH-SY5Y whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 21 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片2
    Staining STMN2 in Human brain tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片3
    Staining STMN2 in Human liver tissue sections by Immunohistochemistry (IHC-P - Fixative-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

    产品细节图片4
    Immunofluorescent analysis of 4% Fixative-fixed, 0.1% Triton X-100 permeabilized SH-SY5Y (Human metastatic neuroblastoma cell line) cells labeling STMN2 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasm on SH-SY5Y cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin at 1/100 dilution (red).

    产品细节图片5
    Overlay histogram showing SH-SY5Y cells stained (green line). The cells were fixed with 4% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (, 1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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    ¥2106 - 3861