alpha Tubulin Antibody 抗体,orb1925729,Biorbyt产品图

alpha Tubulin Antibody 抗体,orb1

925729,Biorbyt
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  • ¥2106 - 3861
  • Biorbyt已认证
  • orb1925729
  • 英国
  • 2026年08月07日
  • FC, IF, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      alpha Tubulin Antibody 抗体

    • 抗体英文名

      alpha Tubulin Antibody

    • 靶点

      TUBA1B

    • 应用范围

      FC, IF, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb1925729

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      50 ul/100 ul

    规格:50 ul产品价格:¥2106.0
    规格:100 ul产品价格:¥3861.0
    类别: Antibodies

    描述: Purified Rabbit Polyclonal Antibody (Pab)

    别名: Tubulin alpha-1B chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-ubiquitous chain, TUBA1B

    应用稀释比例: IF - 1:25, WB - 1:2000, FC - 1:25

    免疫原: This alpha Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 408-439 amino acids from human alpha Tubulin.

    分子量: 50152 Da

    预测反应性: C. elegans, Hamster, Mouse, Porcine, Rat

    研究领域: Cell Biology

    UniProt ID: P68363

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

    备注: For research use only.

    产品细节图片1
    Immunofluorescent analysis of 4% Fixative-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling alpha Tubulin at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. The nuclear counter stain is DAPI (blue).

    产品细节图片2
    All lanes: Anti-alpha Tubulin Antibody at 1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: Jurkat whole cell lysate. Lane 6: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

    产品细节图片3
    Overlay histogram showing Hela cells stained (green line). The cells were fixed with 2% Fixative (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

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    图标文献和实验
    相关实验
    • Immunofluorescent Localization of Tubulin

      antibody to tubulin) FITC-labeled goat anti-rabbit (or secondary antibody to match the primary) Fluorescence Mounting Media Fluorescent Microscope equipped with 490 nm excitation filter and 515 nmbarrier filter Kodachrome film

    • Detection of Antibodies to Self-Antigens (K-alpha 1 Tubulin, Collagen I, II, IV, and V, Myosin, and Vimentin) by Enzyme-Linked Immunosorbent Assay (EL

      . Here, we describe the detection of autoAbs to various self-antigens with different tissue restriction patterns which includes collagens, k-α1 tubulin, vimentin, and myosin. We also discuss their relevance in monitoring for rejection following solid organ transplantation.

    • 靶点星选|全细胞内参β-Tubulin

      、小鼠的β-Tubulin蛋白,其应用亦经猴、鸡、兔、猪、牦牛、果蝇以及斑马鱼等样本证实,能全面覆盖多种实验模型的需求。在应用性能上,该抗体经多种关键技术验证,包括Western Blot(WB)、免疫组化(IHC)、免疫荧光(IF)和流式细胞术(FCM),均表现出色,呈现清晰特异的信号与洁净的背景,有力保障实验数据的可靠性与可重复性。 产品展示 1.K200059M Anti-β-Tubulin Monoclonal antibody 抗体类型:鼠单抗 验证图片:   2.K106386P

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