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- ￥7254
- Biorbyt
- orb1821571
- 英国
- 2026年08月06日
- ELISA, IHC, IP, WB
- Mouse
- Human
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- 技术资料
- 抗体名：
Anti-Cytokeratin 8 [TS1] 抗体
- 抗体英文名：
Anti-Cytokeratin 8 [TS1]
- 靶点：
Cytokeratin 8
- 浓度：
1 mg/ml
- 应用范围：
ELISA, IHC, IP, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1821571
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG
- 免疫原：
详询
- 规格：
0.2 mg
类别: Antibodies
描述: Anti-Cytokeratin 8 [TS1]
克隆号: TS1
别名: CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; TS 1; Mab 202
免疫原: The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
防腐剂: PBS with 0.02% Proclin 300.
特异性: This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340–365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
研究领域: Cancer Biology, Protein Biochemistry
UniProt ID: P05787
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
描述: Anti-Cytokeratin 8 [TS1]
克隆号: TS1
别名: CK8; CK-8; CK 8; KRT8; CYK8; Keratin type II cytoskeletal 8; Cytokeratin-8; Type-II keratin Kb8; TS 1; Mab 202
免疫原: The original antibody was generated by immunizing BALB/c mice with purified human gastrointestinal tumor derived cytokeratin.
防腐剂: PBS with 0.02% Proclin 300.
特异性: This antibody binds a discontinuous epitope on helical 2B domain of the human cytokeratin 8 between amino acid residues 340–365 (QRGELAIKDANAKLSELEAALQRAKQ). Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Together with KRT19, cytokeratin 8 helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin 8 is a serological tumor marker
研究领域: Cancer Biology, Protein Biochemistry
UniProt ID: P05787
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Flow cytometry using anti-Cytokeratin 8 antibody TS1. Fixative fixed HeLa cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of TS1 (orb1821571, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.
Immunofluorescence staining of HeLa cells with anti-Cytokeratin 8 antibody TS1. Immunofluorescence analysis of Fixative fixed HeLa cells, permeabilized with 0.15% Triton, on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TS1 (orb1821571) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb1821571, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.
Western blot using anti-Cytokeratin 8 antibody TS1. A431 cells lysate (35 µg protein in RIPA buffer) was resolved on an SDS-PAGE gel and blots were probed with the chimeric rabbit version of TS1 (orb1821571) at 0.3 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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Anti-Cytokeratin 8 [TS1] 抗体，orb1821571，Biorbyt
￥7254
