Glucocorticoid Receptor/NR3C1 Rabbit Polyclonal Antibody 抗体，orb251525，Biorbyt产品图

Glucocorticoid Receptor/NR3C1

Rabbit Polyclonal Antibody 抗体，orb251525，Biorbyt
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  • ￥5499
  • Biorbyt已认证
  • orb251525
  • 英国
  • 2026年07月22日
  • IHC, KO/KD Validated, WB
  • Rabbit
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Glucocorticoid Receptor/NR3C1 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名：

      Glucocorticoid Receptor/NR3C1 Rabbit Polyclonal Antibody

    • 靶点：

      Glucocorticoid receptor

    • 浓度：

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围：

      IHC, KO/KD Validated, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      12 months from date of receipt.

    • 抗原来源：

      详询

    • 目录编号：

      orb251525

    • 级别：

      科研

    • 库存：

      99

    • 供应商：

      Biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 保存条件：

      详询

    • 形态：

      Lyophilized

    • 亚型：

      Rabbit IgG

    • 免疫原：

      详询

    • 规格：

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Glucocorticoid Receptor/NR3C1 Rabbit Polyclonal Antibody

    别名: Glucocorticoid receptor; GR; Nuclear receptor subfamily 3 group C member 1; NR3C1; GRL

    应用稀释比例: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

    免疫原: E.coli-derived human NR3C1 recombinant protein (Position: A20-F199). Human NR3C1 shares 80% and 74% amino acid (aa) sequence identity with mouse and rat NR3C1, respectively.

    交叉反应: No cross-reactivity with other proteins

    分子量: 100 kDa

    防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

    特异性: No cross reactivity with other proteins.

    研究领域: Cell Biology, Epigenetics & Chromatin, Signal Transduction

    UniProt ID: P04150

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-NR3C1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

    产品细节图片2
    IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-NR3C1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

    产品细节图片3
    IHC analysis of NR3C1 using anti-NR3C1 antibody. NR3C1 was detected in a paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-NR3C1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

    产品细节图片4
    Western blot analysis of NR3C1 using anti-NR3C1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NR3C1 at approximately 100 kDa. The expected band size for NR3C1 is at 86 kDa.

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