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- IBL-international
- REHM300
- 2025年07月14日
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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
mixing as this will shear chromosomal DNA and lower plasmid purity. The lysate should appear viscous. Do not allow the lysis reaction to proceed more than 5 min. (Store Buffer T2 tightly capped when not in use). 5. Add 200 ul of Chilled Buffer T
BACs, PACs, P1s and Plasmid Isolation Protocol (For low copy number)
to obtain a clear lysate. Incubate at room temperature for 5 minutes. Avoid vigorous mixing as this will shear chromosomal DNA and lower plasmid purity. The lysate should appear viscous. Do not allow the lysis reaction to proceed more than 5 min. (Store
即肽酰tRNA位点(peptidyl-tRNA site), 又叫供位(donor site), 或肽酰基位点, 主要位于大亚基, 是肽基tRNA移交肽链后肽酰tRNA所占据的位置, 即与延伸中的肽酰tRNA结合位点。
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