MTA2 Rabbit Monoclonal Antibody 抗体,orb548157,Biorbyt产品图

MTA2 Rabbit Monoclonal Antibod

y 抗体,orb548157,Biorbyt
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  • ¥5499
  • Biorbyt已认证
  • orb548157
  • 英国
  • 2026年07月22日
  • FC, ICC, IF, IHC, WB
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      MTA2 Rabbit Monoclonal Antibody 抗体

    • 抗体英文名

      MTA2 Rabbit Monoclonal Antibody

    • 靶点

      Metastasis-associated protein MTA2

    • 浓度

      0.5mg/ml

    • 应用范围

      FC, ICC, IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb548157

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 保存条件

      详询

    • 形态

      Liquid

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      100 ul

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-MTA2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

    克隆号: BCEQGX35

    别名: TNF receptor-associated factor 4; Cysteine-rich domain associated with RING and Traf domains protein 1; Metastatic lymph node gene 62 protein; MLN 62; RING finger protein 83; TRAF4; CART1, MLN62, RNF83

    应用稀释比例: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 FC 1:50

    免疫原: A synthesized peptide derived from human MTA2 The p53 tumor suppressor gene integrates numerous signals that control cell life and death. There are several proteins that are involved in the p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and MTA2. The transcriptional activity of p53 is modulated by protein stability and acetylation.

    分子量: 75 kDa

    防腐剂: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

    纯化方法: Affinity-chromatography

    研究领域: Epigenetics & Chromatin, Immunology & Inflammation

    UniProt ID: O94776

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of MTA2 using anti-MTA2 antibody (orb548157) in HeLa cell lysate. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTA2 antigen affinity purified polyclonal antibody (Catalog # orb548157) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system.

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    图标文献和实验
    相关实验
    • Generation and Selection of Rabbit Antibody Libraries by Phage Display

      The rabbit antibody repertoire is an exceptional source for both polyclonal antibodies (pAbs) and monoclonal antibodies (mAbs) that combine high specificity with high avidity and affinity, respectively. In contrast to rabbit pAbs

    • Polyclonal Antibody Production

      , if desired this is best accomplished by Protein A Affinity Chromatography.   上一篇:make an Antibody against a Peptides   下一篇:Immunization Protocol for Monoclonal Antibody Production

    • Isoelectric Focusing and Immunofixation of Monoclonal Antibodies

      Isoelectric focusing of monoclonal antibodies in agarose gel is one of several ways to characterize a hybridoma. Isoelectric focusing is performed on each monoclonal antibody, and the mouse immunoglobulin bands are immobilized by soaking

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