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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
family (4 ), however, in vitro DNA-PK acts as a serine/threonine protein kinase (5 –3 ). The physiological substrates of DNA-PK are not known, however in vitro DNA-PK is known to phosphorylate many proteins including hsp90, SV40 large T antigen, serum
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
Use of Animal Models to Understand Human Muscular Dystrophy
, respectively; laminin α2 -chain mutations have been shown to result in congenital MD (4 ). Defects in non-DGC components that cause include the calpain 3 in LGMD2A (4 ), expansion of a CTG repeat in the 3′ untranslated region of a serine threonine kinase
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