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文献和实验Recombinant DNA Engineering, Or Cloning Genes In Plasmids
also usually have promoter segments for phage polymerases (like T7 and SP6 RNA polymerases) such that one can use in them vitro to produce RNA. A plasmid designed for mammalian expression also usually has a transcription termination and polyadenylation signal
In this chapter we describe efficient procedures for the construction, expression and screening of comprehensive libraries of human Fab antibody fragments displayed on the surface of filamentous phage. Phagemid vectors are used for placing
Highly Purified Recombinant Adeno-Associated Virus Vectors: Preparation and Quantitation
Adeno-associated virus (AAV) is a nonpathogenic, replication-defective parvovirus that is being developed as a vector for human gene transfer. The recent interest in recombinant (r)AAV has been driven by the unexpected finding
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