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Neobioscience
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文献和实验and p75) (2 ). Signaling through the p55 receptor is primarily associated with responses such as cytotoxicity (2 ,3 ) and cytokine secretion (4 ) whereas the p75 receptor is responsible for lymphoproliferative signals and the activation of T-cells
Detection of TNF and TNF Receptor mRNA in Cells and Tissues
We describe a semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique for the quantification of the messenger RNA of human and murine tumor necrosis factor α (TNFα) and related receptors. This protocol can be adapted
Production and Characterization of Receptor-Specific TNF Muteins
receptors (TNFR) (radioligand competition binding and Biacore). The biological activity of wild-type TNF and/or TNF muteins can be assessed in cellular assays. TNF-induced cytotoxicity toward murine L929s cells and human Kym39A6 cells is mediated
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