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文献和实验Site-Directed Mutagenesis of Antibody-Variable Regions
oligonucleotide-directed mutants. Several polymerase chain reaction-based mutagenesis techniques have been described (3 –5 ). However, available thermostable polymerases are error-prone, leading to undesired sequence changes. For example, Taq polymerase
DNA-Directed Assembly Microarray for Protein and Small Molecule Inhibitor Screening
. Herein, we describe the detailed experimental protocols in performing the DNA-directed assembly platform for antibody microarray, a RNA polymerase-DNA binding microarray, and a drug-screening microarray.
Introduction The recent discovery of RNA interference and in particular the observation that siRNAs can modulate gene expression at the level of transcription, i.e. small-interfering RNA (siRNA) directed transcriptional gene silencing (TGS) in Human
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