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- 2025年07月14日
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文献和实验【申请上传】Current Protocols in Immunology
ELISA Unit 7.13 Measurement of Polyclonal Immunoglobulin Synthesis Using the Reverse Plaque Technique Unit 7.14 Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay Unit 7.15 Measurement of In Vitro Specific-Antibody Synthesis Unit 7.16
of action To prove that the in vivo activity was due to siRNA-directed cleavage, we characterized specific mRNA cleavage products using a modified 5'-RACE (rapid amplification of cDNA ends) technique previously used to demonstrate microRNA (miRNA)-directed
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
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