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- 详细信息
- 文献和实验
- 技术资料
- 克隆性:
1A8
- 标记物:
PE-Cy7
- 规格:
25 ug
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文献和实验Western Blotting with Horseradish Pe
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10–15 seconds
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
Anti-factor Xa (Anti-Xa) Assay
The anti-factor Xa (anti-Xa) assay is a functional assay that facilitates the measurement of antithrombin (AT)-catalyzed inhibition of factor Xa by unfractionated heparin (UFH) and direct inhibition of factor Xa by low-molecular-weight
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