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- BioGems
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- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 克隆性：
HRPN
- 标记物：
FITC
- 规格：
100 ug
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文献和实验Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
on mouse tissue are of IgG1 isotype. Detailed explanation of this method can be found in: Cattoretti G, Qing Fei. Application of the Antigen Retrieval Technique in experimental Pathology: from human to mouse
to the injection procedure and being on the maze in order to control for these effects. Note: always wear rubber gloves & lab coat. 1. Prepare saline injection ~ (.2 ml.). 2. Give the rat an interperitineal injection. 3. Repeat process
Immunofluorescent Staining of Mouse and Rat Leukocytes
secondary reagent (e.g., fluorochrome-conjugated avidin, streptavidin, anti-Ig allotype, anti-Ig isotype, polyclonal anti-Ig). For example, dilute antibody to ~1 µg per 100 µl in wash buffer and add this to each well containing the loosened cell pellet
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