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- 克隆性：
104
- 标记物：
APC
- 规格：
25 μg
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文献和实验Purification Of Anti Peptide Antibody
Protocol: All steps up to the dialysis at rt. Pour column in TBS (=0.15M NaCl, 20mM TrisCl pH 7.4). We use a 5 ml column for 25 mls serum. Wash extensively in TBS after prewashing as indicated in the protocol for coupling
Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
, I clean (with 70% alcohol) the following (all Roboz instruments): 5 pairs of forceps 1 pair Pattern 3c 1 pair straight serrated, 3.5" 1 pair curved serrated, 3.5" 1 pair of 3.5" sharp / sharp scissors (Surecut) 25 guage
Anti-GFP/BrdU In Situ Immunostaining
Materials 24 well dishes Millicell 0.4 µm inserts (Millipore PICM01250) - coated with fibronectin Culture medium PBS Fixative: 4% paraformaldehyde in PBS (pH 7.6) Rabbit anti-GFP (Molecular Probes, A-11122) FITC-goat anti-rabbit
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