- ￥585
- 华韵生物(Huayunbio)
- HFC2918-25μg
- 2025年07月15日
- FCM
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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 供应商：
广州华韵生物科技有限公司
- 应用范围：
FCM
- 规格：
25μg
请联系华韵客服获取说明书。
产品简介：
| Background |
CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
|
| Alternate Names |
LCA;Ly-5.2
|
| Gene ID |
19264
|
| Clone No |
104.2
|
| Host |
Mouse
|
| Reactivity |
Mouse
|
| Application |
FCM
|
| Isotype |
Mouse IgG2a, κ
|
| Isotype Control |
APC Mouse IgG2a, κ Isotype Control[C1.18.4]
|
| Conjugation |
APC
|
| Conjugation Information |
APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter).
|
| Spectrum |
|
| Storage Buffer |
Phosphate buffered solution, pH 7.2, containing 0.09% sodium azide and 1% BSA.
|
| Storage |
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
|
| Expiration date |
12个月
|
| Shipping |
冰袋
|
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文献和实验分泌。 随后进行表面染色：先以Fc阻断剂（2.4G2克隆，5 μg/mL）处理10分钟，再于4°C避光孵育15分钟，使用含1% BSA和0.1% NaN₃的DPBS缓冲液配制抗体，常用组合包括CD90.2、CD4、CD8、CD25、CD11b和CD45.2等标记。 固定步骤采用2%甲醛（FA，由含<3%甲醇的4% Roti-Histofix稀释），室温孵育40–60分钟。破膜与胞内染色使用0.1%皂苷缓冲液或1×Perm缓冲液，加入抗FoxP3、T-bet、RORγt、IL-17A或IFN-γ等抗体，4°C
Diego, CA); GSK-3α (Upstate, LakePlacid, NY); and b-actin (Sigma, St. Louis, MO). 7. Secondary antibodies: anti-mouse and anti-rabbit IgG conjugatedto horseradish peroxidase (Santa Cruz Biotechnology,Santa Cruz, CA). 8. Enhanced chemiluminescent
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
: anti-mouse or anti-rabbit conjugated tobiotin from Dako Corp. (Glostrup, Denmark) diluted 1:500. 8. Third antibody: Texas Red conjugated to avidin from VectorLaboratories (Burlingame, CA) diluted 1:500. 9. Nuclear stain: 0.05 μg/mL of Hoechst
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