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文献和实验Intracellular Cytokine Staining Protocol
-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used.Note: Optimal
Models of Inflammation: Carrageenan Air Pouch
:5.6.1?5.6.8. © 2012 by John Wiley & Sons, Inc. Keywords: inflammation; edema; carrageenan; air pouch; animal model; inflammatory mediator; PGE2; TNF alpha
Intracellular Cytokine Staining Protocol
to produce IFN-gamma, TNF-alpha, IL-2, and IL-4, a combination of PMA (a PKC activator) and Ionomycin (a calcium ionophore) or anti-CD3 antibodies are used. To induce IL-6, IL-10 or TNF-alpha production by monocytes, stimulation of PBMC's with LPS is used
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