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- BioGems
- 07512-70-100
- 2025年07月07日
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- 文献和实验
- 技术资料
- 克隆性：
30-F11
- 标记物：
PerCP-Cy5.5
- 规格：
100 ug
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文献和实验Cyanine Dye Purification Protocol
.0 . Add 80 mL acetonitrile (HPLC grade) Adjust the final volume to 800 mL with ddH2 O. LSB, 1000 mL To 25 mL HSB , add 100 mL HPLC grade acetonitrile Raise final volume to 1L with ddH2 O
40, then again with PBS/0.1 BSA. Mount solution (70 glycerol containing 2 n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20oC until examination. Antibodies mouse
Anti-HA Immunocytology The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots
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