- ￥930 - 2320
- Proteintech
- CPY5-65108
- 2025年10月09日
- FC
- Mouse
- human
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG2b, kappa
- 保存条件：
Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.
- 克隆性：
Monoclonal
- 标记物：
PerCP-Cyanine5.5 Fluorescent Dye
- 适应物种：
human
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD45RA
- 抗体英文名：
PerCP-Cyanine5.5 Anti-Human CD45RA (HI100)
- 抗体名：
PerCP-Cyanine5.5 Anti-Human CD45RA (HI100)
- 规格：
25tests/100tests
| 规格： | 25tests | 产品价格： | ￥930.0 |
|---|---|---|---|
| 规格： | 100tests | 产品价格： | ￥2320.0 |
经过测试的应用
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CPY5-65108 targets CD45RA in FC applications and shows reactivity with human samples.
| 经测试应用 | FC |
| 经测试反应性 | human |
| 免疫原 |
fusion protein |
| 宿主/亚型 | Mouse / IgG2b, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | protein tyrosine phosphatase, receptor type, C |
| 别名 | CD45, EC:3.1.3.48, GP180, HI100, L CA |
| GenBank蛋白编号 | BC014239 |
| 基因名称 | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_3673819 |
| 偶联类型 | PerCP-Cyanine5.5 Fluorescent Dye |
| 最大激发/发射波长 | 482 nm / 690nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | P08575 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).
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文献和实验; PBMC are collected and sorted according to surface marker expression. Cell separation can be achieved by fluorescence-activated cell sorting (FACS) using CD4, CD45RA and CD25 to define memory Treg (CD4+ CD25hi ), or by a combination of magnetic bead
Mouse anti-Human CD45 mAb,Biotin 100ug/500ug 800/2500 L0112 Mouse anti-Human CD45RA mAb,FITC 50T/100T 800/1200 L0113 Mouse anti-Human CD45RA mAb
为分析二抗的使用情况，我们随机抽取了100篇2007年或2008年发表于Science 或 Journal of Biological Chemistry杂志上的文章(英文) ，这些文章涵盖了来自于16个国家的约80个研究机构。收集的数据显示二抗的使用非常广泛，可以应用到几乎所有类型的免疫检测中，尤其是Western blot、免疫组织化学和免疫细胞化学。事实上，二抗在ELISA及流式细胞术中应用也很广泛
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