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文献和实验Obtaining Embryos: hCG Injection
for comments about fertilization rates). The hCG (Chorulon) comes as a powder of 10,000 units that we resuspend in 10 ml of sterile water (final concentration 1 unit/microliter). For the priming dose, we dilute the stock of hCG ten fold and inject 100 µl
is exquisitely sensitive to the process of lipid peroxidation (LP) due to the high content of peroxidizable polyunsaturated acids and a high content of iron which is a catalyst for the propagation of LP reactions in cell membranes. This chapter is focused
) CAUTION: Acrylamide monomer is neurotoxic. A mask should be worn when weighing acrylamide powder. Gloves should be worn while handling the solution, and the solution should not be pipetted by mouth
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