HyperTrap Geldex 75 PG Column-

Column Buffers: CREB

Bases Buffer is Buffer C (as per Schroter)(as per SRF)pg.27 20mM Hepes pH 7.9 0.1% NP40 0.2mM EDTA 20% glycerol Lysis Buffer: 0.75M KCl Need buffers containing 0,0.1M,0.6M & 1.0M KCL in addition to 0.3M,1.5M & 2.0 used in SRF purification pg

Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF

standardsNgf Standards IIPg NGF/100 µlAddSample Buffer +0.1%tw10030 µl of #23 ml5015 µl of #23 ml2575 µl of #2.53 ml12.537.5 µl of #2.53 ml6.2518.75 µl of #2.53 ml3.1293.6 µl of #33 ml1.5646.8 µl of #33 ml• PROTEIN RECOVERYIs determined by adding a known

下游纯化工艺简介

过滤法方法简单、重现性强，目前广泛应用。 凝胶过滤具有很多不同分离范围的凝胶系列供选择。 要点: 采用细长（L/D~20）的柱、选择合适的凝胶、上样体积较小、流速较慢。 以下为常用凝胶过滤用凝胶及其基本参数 系列 名称 分离范围 颗粒大小 应用 Superdex prep grade系列 Superdex 30PG 小于10000 24~44微米 小肽 Superdex 75PG 3000~70000 24~44微米 一般蛋白 Superdex 200PG 10000~600000 24~44