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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
储存于-20 ° C or below
- 保质期:
1年
- 英文名:
Aminoallyl-dUTP
- 库存:
100
- 供应商:
艾美捷科技
- CAS号:
无
- 规格:
1ml (10mM)
Aminoallyl-dUTP,Aminoallyl-dUTP,Aminoallyl-dUTP,Aminoallyl-dUTP
Aminoallyl-dUTP-Aminoallyl-dUTP
产品货号:APE-B7950-51
产品规格:1ml (10mM)
通过PCR标记DNA / cDNA,并用作反应性位点,用于随后的反应性荧光探针标记。

Modified Nucleotides

Aminoallyl-dUTP-Aminoallyl-dUTP储存于-20 ° C or below
点击:Aminoallyl-dUTP-Aminoallyl-dUTP查看更详细产品说明,更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多APExBIO公司特色小分子抑制剂等产品评论,请点击查看艾美捷特色产品中心。
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文献和实验[原理] DNA是通过异羟基洋地黄毒苷(digoxigenin,Dig)配基标记的脱氧尿嘧啶核苷三磷酸(dUTP)随机插入结合而被标记。dUTP通过间臂连结类固醇半抗原异羟基洋地黄毒苷酸基,形成Dig-dUTP,杂交反应后,杂交的靶DNA通过酶联免疫法与一个抗体复合物[抗异羟基洋地黄毒苷配基碱性磷酸酶复合物(Dig)Ap]结合,接着在5-溴-4氯-3-吲哚磷酸盐(X-磷酸盐)和硝基四氮唑蓝(NBT)存在下,由酶催化反应,在杂交部位形成蓝紫色带或颗粒。 [操作] 1.转移 通过打点吸渍
Random Prime Labeling of DNA Probes with Fluorescein-11-dUTP
The selection of an appropriate labeling reagent for a particular experiment, for the most part, depends on the sensitivity and resolution required. For maximum sensitivity in filter hybridizations radioactive labels, such as phosphorous 32
Detection of Apoptosis in Atherosclerosis and Restenosis by Terminal dUTP Nick-End Labeling (TUNEL)
Kerr and Wyllie (1 ) have introduced the term apoptosis to separate this special form of cell death from necrosis. When a cell receives a signal to die an apoptotic death, it goes through a series of morphological changes that can be easily
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