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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
咨询
- 英文名:
Aminoallyl UTP Solution
- 库存:
大量
- 供应商:
宝柏·中国
- 规格:
50 ul
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文献和实验Revised amino-allyl labeling protocol
1.5 0.5 50X dNTP stock solution using a 4:1 ratio aminoallyl-dUTP to dTTP***: 10 m L each 100 mM dATP, dGTP, dCTP (Pharmacia
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
DTT Superscript II RT (Gibco) RNasin (Gibco, optional) 0.1M 200U/mL 40U/mL 6.0 mL 0.6 3.0 1.5 0.5 50X dNTP stock solution using a 4:1 ratio aminoallyl-dUTP to dTTP***: 10 mL each 100 mM
Protocol for Trichloroacetic Acid (TCA) Precipitation
of radiolabeled nucleotide incorporation in a polymerization reaction by TCA precipitation. In the protocol, nucleic acid synthesis reactions are carried out in the presence of a radiolabeled nucleotide (e.g., 32 P-dATP or 32 P-UTP). Then, the synthesized polymers
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