Thonzonium Bromide-Thonzonium

Ethidium bromide

  Introduction   Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods

Ethidium Bromide Decontamination

N.B.: Ethidium bromide is a powerful mutagen. Protective gloves should be worn at all times when handling solutions containing ethidium bromide.   Decontamination of solutions > 0.5mg/ml This method reduces the mutagenic activity

Clean-up for Ethidium Bromide

Caution: EtBr is a powerful mutagen and is moderately toxic. Wear gloves when working. When EtBr conc. is >0.5 mg/ml: 1.Add sufficient water to reduce the concentration of EtBr to 2.Add 1 volume of 0.5 M KMnO4. Mix, add 1 volume of 2.5 N HCl