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- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
/
- 肿瘤类型:
/
- 细胞类型:
/
- 品系:
/
- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
/
- 物种来源:
人或动物
- 免疫类型:
/
- 细胞形态:
/
- 是否是肿瘤细胞:
/
- 器官来源:
/
- 运输方式:
常温或干冰
- 年限:
/
- 生长状态:
/
- 规格:
T25
|
种属 |
小鼠 |
|
组织来源 |
正常关节组织 |
|
传代比例 |
1:2传代 |
|
完全培养基配置 |
基础培养基500ml ;生长添加剂5ml ;胎牛血清50ml ;双抗5ml |
|
简介 |
滑膜是关节囊的内层 ,淡红色 ,平滑闪光 ,薄而柔润 ,由疏松结缔组织组成。关节腔内的所有结构 ,除关节软骨、半 月软骨板以外,即便是通过关节腔的肌腱、韧带等均全部为滑膜所包裹。 关节软骨一旦损伤将很难修复 ,运用自体软骨细胞移植技术治疗软骨损伤 ,效果较好 ,但由于细胞来源不足以及取材 造成的取材部位发生病变等因素 ,使其应用受到限制 ,间充质干细胞( MSCs )具有高度增殖和多向分化潜能的特 性。其中 ,滑膜中间充质干细胞的比例较高 ,少量的标本就可获得足量的滑膜MSCs。此外 ,由于其具有比骨髓、脂 肪等来源的MSCs更好的成软骨、成骨、成脂肪等多分化能力和更强的集落形成能力等吸引了许多研究者对其进行深 入研究。 |
|
形态 |
成纤维样细胞样 |
|
生长特征 |
贴壁生长 |
|
细胞检测 |
CD105免疫荧光染色为阳性免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、HCV、支原体、细菌、 酵母和真菌等。 |
|
倍增时间 |
每周 2 至 3 次 |
|
换液频率 |
2-3天换液一次 |
|
培养条件 |
气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
|
冻存条件 |
冻存液 :90%FBS ,DMSO 10%, 或使用非程序冻存液 :官网货号JY-H040 |
|
产品使用 |
仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |
abundant desmoplastic reaction, may profoundly affect the proliferative and invasive abilities of
Conclusions: The CD103+ CD39+ CD8+ cell change after administration may predict the e ff icacy of ICIs.PURPOSE: There is increasing effort to discover and integrate predictive and/or prognostic with TMB. Immune-related differentially expressed genes (irDEGs) were identified. Hub irDEGs independently related to prognosis were analyzed using univariate and multivariate Cox proportional hazard models. A survival signature was constructed from hub irDEGs.play a pivotal role in cancer management plans when used alongside medical, surgical, and radiation oncology in the care of cancer patients.Tumor associated macrophages (TAMs) are important components residing in the tumor microenvironment. They are immunosuppressive SBRT target region for the bulky tumor are warranted.The gut microbiota is a large symbiotic community of anaerobic and facultative aerobic bacteria inhabiting the human intestinal tract,
Objective: The objective was to assess the roles of 68Ga-PSMA PET/CT and 18F-NaF PET/CT in evaluation of skeletal metastatic lesions in prostate cancer.
Methods: Two hundred consecutive prostate cancer patients who had undergone 68Ga-PSMA PET/CT and 18F-NaF PET/CT at baseline evaluation (n = 80) and following suspected recurrence or disease progression (restaging) (n = 120) were analyzed retrospectively.
Results: PSMA and NAF scans were positive for skeletal metastatic lesions in 67% (134 patients) and negative in 33% (66 patients). The scans were concordant in 80% (160 patients: 66 negative and 94 positive) and discordant in 20% (40 patients). Among 40 discordant results, 14 were baseline and 26 were restaging studies. PSMA detected more number of lesions in 11 (nine baseline and two restaging). These were true positive marrow or lytic metastatic lesions. NaF revealed more number of lesions in 29 (5 initial and 24 restaging). These were false positive on follow-up imaging. No statistical difference (P value = 0.7 by McNemar test) between the two scans for identifying absence or presence of at least one skeletal lesion was noted at baseline staging.
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文献和实验/biomarkers into treatment algorithms. While tissue-based methods can reveal tumor-immune cell compositions at a single time point, we propose that single-cell sampling via fine needle aspirates (FNA) can facilitate serial assessment of the tumor immune microenvironment (TME)
一、细胞复苏和接种 1. 37℃预热小鼠间充质干细胞培养液和基础培养液。 2. 从液氮中取出细胞产品管,快速将其置入37℃水浴中解冻,直到管中只剩下最后一片结晶(注意:不要让水没过产品管)。 3. 在生物安全柜中,用75%的酒精擦拭产品管外壁。 4. 将产品管中的细胞移至15ml无菌离心管中,慢慢逐滴加入预温的37℃基础培养液4-5 ml混匀,边滴加边摇匀。 5. 用1ml基础培养液冲洗产品管,并将其转移到15 ml离心管中,边滴加边摇匀。 6. 轻轻混匀
细胞,如巨噬细胞、单核细胞、中性粒细胞和树突状细胞。 虽然移植的细胞在体内大部分都已解体,但它们的细胞残余可以通过血液运输到身体的其他部位,并在那里它们被吞噬。移植细胞的清除使吞噬细胞向抗炎、耐受性和再生表型极化,事实上,这些清除死亡细胞的重编程吞噬细胞可以在全身迁移,直接或通过改变免疫抑制或抗原呈递机制来调节适应性免疫系统的细胞,从而进一步介导组织修复和疾病部位的再生。例如,静脉输注的小鼠骨髓间充质干细胞可以通过极化内源性巨噬细胞产生抗炎细胞因子白细胞介素 - 10 (IL-10) 来减轻小鼠的败血
中国科学家团队合作发现促进多组织再生、延缓衰老的小分子代谢物
)以及年轻和衰老的间充质干细胞,系统揭示了一些跨物种保守的、再生相关的代谢通路。例如,再生能力强的生物样本更倾向于富集多胺代谢、尿嘧啶代谢和脂肪酸代谢通路。 进一步结合人类干细胞衰老的研究平台,研究人员对潜在的促再生代谢物进行了细致的筛选,发现小分子代谢物尿苷(Uridine)可以明显提升衰老人间充质干细胞的自我更新能力。 进一步研究表明,尿苷处理可以在 5 种小鼠的组织损伤模型(肌肉损伤模型、肝纤维化模型、毛发再生模型、心肌梗塞模型和关节炎模型)中助力损伤或病变组织的再生修复。在肌肉损伤模型中,尿苷
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