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- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
/
- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
/
- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
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- 生长状态:
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- 规格:
T25
| 种属 | 人 |
| 组织来源 | 正常牙组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清10ml ;双抗5ml |
| 简介 | 牙龈是附着在牙颈和牙槽突部分的粘膜组织 ,呈粉红色 ,有光泽 ,质坚韧 ,牙龈成纤维细胞是牙龈结缔组织中的主要 细胞 ,不仅具有活跃的自身更新能力 ,而且可合成胶原纤维、弹性纤维及机制 ,在牙周组织的保护和修复中起重要作 用。 |
| 形态 | 成纤维样细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 纤维连接蛋白( Fibronectin )免疫荧光染色为阳性免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、 HCV、支原体、细菌、酵母和真菌等。 |
| 倍增时间 | 每周 2 至 3 次 |
TLR2ofthe women withGDMareincreasedabnormally , andbothofwhichareclosely relatedtothefunctionofislet cellsandtheadversepregnancy outcomesofthese women.
Keywords Gestationaldiabetesmellitus ; SolubleCD14 ; Toll-likereceptor2; Isletcellfunction ; Adversepregnancy outcomes ; Influencefactor
cells in draining LNs derived from kCYC mice bearing B16 melanoma also showed significantly decreased cytotoxic activity in vitro. Finally, tumor suppression activity of CD8+ T cells derived from kCYC mice bearing B16 melanoma was reduced when adoptively transferred to naive wild-type mice. In summary, these findings suggest that lymphatic transport is essential in
An increased white blood cell count, or leukocytosis, is a common laboratory finding. Appropriate specimen evaluation depends on which lineages are increased and the morphologic findings on peripheral blood smear review to guide further testing. The presence of blasts is concerning for acute leukemia and may require bone marrow biopsy. Lymphocytosis may be morphologically divided into polymorphic and monomorphic populations. Polymorphic lymphocytosis is most consistent with a reactive process, while monomorphic populations are concerning for lymphoproliferative neoplasm. The differential can be further narrowed based on morphologic findings. Myeloid leukocytosis can occur in a number of reactive conditions as well as myeloid malignancies. The types of cells present and morphology can help to guide additional workup. This study provides guidance for the appropriate evaluation and further workup of leukocytosis.
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文献和实验/TLR2ofthe women withGDMareincreasedabnormally , andbothofwhichareclosely relatedtothefunctionofislet cellsandtheadversepregnancy outcomesofthese women.
Keywords Gestationaldiabetesmellitus ; SolubleCD14 ; Toll-likereceptor2; Isletcellfunction ; Adversepregnancy outcomes ; Influencefactor
口腔颌面部的恶性肿瘤以癌最常见,在癌瘤中又以鳞状细胞癌为最多见,在我国,口腔颌面部鳞癌多发生于40-60岁之间,男性多于女性,以牙龈癌、舌癌、颊癌、腭癌以及上颌窦癌常见,口腔癌在我国长江以北,占全身恶性肿瘤的1.45-5.6%,长江以南为1.75-5.18%。在印度其在全身恶性肿瘤中高达40%以上,按照病理分化程度,鳞癌一般可分为Ⅲ级:Ⅰ级分化程度高,恶性程度低;Ⅲ级分化差,恶性程度高;未分化癌的恶性程度最高,牙龈癌在我国发生率较高,据上海第二医科大学的统计资料,其在恶性
丁香园网友hyong915的观点为:成纤维细胞培养(一) 原代培养1、在手术室无菌条件下,切取新鲜的皮肤,增殖性瘢痕和瘢痕疙瘩组织,修除表皮和皮下组织,盐水反复冲洗后放入含PS的DMEM培养液内带回无菌工作间。2、把组织块置于培养皿内,Hank,s液漂洗三遍后吸净Hank,s液,眼科剪反复剪切组织块成0.5-1mm3大小。用弯头吸管吸取组织块接种于40ml培养方瓶瓶壁上,组织块间留约0.3-0.5cm的间距。3、 塞好瓶塞,放入37℃电热恒温培养箱内3.5小时使培养的组织小块微干涸
liupeizc 请问哪位高手知道成纤维细胞的生长周期啊,更确切的是血管外膜成纤维细胞生长周期,谢谢! zhujoker 估计都没人做过,你如果需要观察其生物学功能,就自己做一次,也算原创了啊! freecell 这里有: http://www.currentprotocols.com/protocol/cb0201 本文由丁香园论坛提供,想了解更多有用的、有意
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