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- 文献和实验
- 技术资料
- 英文名:
/
- 库存:
现货库存
- 供应商:
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- 肿瘤类型:
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- 细胞类型:
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- 品系:
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- 组织来源:
ATCC/DSMZ/ECACC
- 相关疾病:
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- 物种来源:
人或动物
- 免疫类型:
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- 细胞形态:
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- 是否是肿瘤细胞:
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- 器官来源:
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- 运输方式:
常温或干冰
- 年限:
/
- 生长状态:
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| 种属 | 人 |
| 组织来源 | 脐带组织 |
| 传代比例 | 1:2传代 |
| 完全培养基配置 | 基础培养基500ml ;生长添加剂5ml ;胎牛血清25ml ;双抗5ml |
| 简介 | 该细胞来源于人静脉内皮 ,可在半固体培养基中形成克隆 ,在免疫抑制小鼠中不能形成肿瘤。 |
| 形态 | 内皮细胞样 |
| 生长特征 | 贴壁生长 |
| 细胞检测 | 血管假性血友病因子( vWF )免疫荧光染色为阳性免疫荧光鉴定 ,细胞纯度可达90%以上 ,不含有HIV-1、HBV、 HCV、支原体、细菌、酵母和真菌等。 |
| 备注 | 该细胞通过慢病毒转染的方式携带RFP基因, ,若要求需要维持荧光强度 ,建议可以加入嘌呤霉素进行再次筛选。 |
Background: Cytokine storm has been used to implicate increased cytokine levels in the pathogenesis of serious clinical conditions. Similarities with Severe Acute Respiratory Syndrome Coronoavirus-2 (SARS CoV-2) and the 2012 Middle Eastern Respiratory Syndrome led early investigators to suspect a cytokine storm resulting in an unregulated inflammatory response associated with the significant morbidity and mortality induced by SARS CoV-2. The threshold of blood cytokines necessary to qualify as a cytokine storm has yet to be defined.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay
innate and acquired immunity against tumors. NK cells and cytotoxic T lymphocytes play a role as effector cells to directly kill tumor cells. On the other hand, antigen-presenting cells, particularly
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文献和实验/Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay
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