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文献和实验Microscopy Imaging Methods for the Detection of Silver and Titanium Nanoparticles Within Cells
chamber slides or collected from T-25 flasks and then fixed in suspension. At the lowest concentrations of TiO2 (0.1–0.3 μg/mL), it was possible to detect as few as 5–10 nanoparticles per cell due to intense light scattering by TiO2 . The degree
SSR GEL and Silver Staining Protocol
.i.). -The washes are done with distilled water. -We use pre-measured sodium thiosulfate (-20℃) and silver nitrate (RT), stored in microtubes. -All incubations and washes are performed at room temperature, but the developer solution must be pre-chilled to 4-10
Silver Enhancement of Colloidal Gold
: Silver lactate, acidic (Danscher, 1981) 0.6 ml gum arabic (33% in bidistilled water) plus 0.1 ml citrate buffer (2.55 g citric acid plus 2.35 g trisodium citrate dihydrate, add bi-distilled water to make 10 ml, pH
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