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文献和实验SSR GEL and Silver Staining Protocol
.i.). -The washes are done with distilled water. -We use pre-measured sodium thiosulfate (-20℃) and silver nitrate (RT), stored in microtubes. -All incubations and washes are performed at room temperature, but the developer solution must be pre-chilled to 4-10
Reverse Transfection Using Gold Nanoparticles
, we have found that the conjugation of gold nanoparticles (20 nm in particle size) to the pEGFP-N1/Jet–PEI complex resulted in an increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal
Silver Enhancement of Colloidal Gold
separately in a freezer. Incubation time for 1-nm gold is about 20 min, for Nanogold about 25 min (20 - 22°C). Silver lactate (Lah et al., 1990) As above, replace citrate buffer by 0.2 M HEPES buffer, pH 6.8. Incubation time ~3 min
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