AE90735Ca 犬S100钙结合蛋白B(S100-B)E

Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b

Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit

Use of B-PER lysis reagent to purify recombinant proteins tagged with His6 or maltose binding protein

were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl

只因没选对它，WB、IF、IHC 统统都白搭....

是否特异性辨认正确蛋白。举例来说，表观遗传学重要蛋白 TET1，一般在细胞内的表达量较低, 蛋白生命周期也短, 往往不易侦测。这时可进一步使用过表达 TET1 的细胞裂解液作对照（下图 B），可看到抗体辨识的条带会明显增强，确定抗体辨识具有特异性。 另一个例子，黑色素瘤标记物-S100B 是钙结合蛋白，主要在中枢神经细胞表达，特别是星形胶质细胞。在黑色素瘤细胞和其他一些组织也发现 S100B 表达。为黑色素瘤的独立预后标志物。GeneTex 的 S100 beta antibody（GTX