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文献和实验Transformation of E. coli by Electroporation电穿孔转化E.coli
. 2.Add 1 mL from overnight culture to 250 mL LB flask; grow until bacteria are in log phase (4-5 hours for E. coli) 3.Split culture across two centrifuge flasks 4.Centrifuge at 2600g for 15 min in a 4 ℃ superspeed centrifuge 5.In cold room or on ice
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
is usually good for 24-36 hours of use). 7. Turn on main power. 8. Turn on main service valves for steam, water and air. 9. Open air, water, and steam valves on fermentor. Check for leaks. 10. Make sure seal steam and seal steam condenser
1.Add colony of desired bacterium to 25 mL LB flask; grow overnight. 2.Add 1 mL from overnight culture to 250 mL LB flask; grow until bacteria are in log phase (4-5 hours for E. coli) 3.Split culture across two centrifuge flasks
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