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文献和实验). 2. Preparation of SIFs and SACS 1) SIFs Preparation SIFs-coated surfaces were formed by an incomplete silver mirror reaction as described earlier (25 ). Glass slides were cleaned by soaking in H2 SO4 (98%) for 10–60 min
. Duringdownwards motion of a squarewave grating (spatial wavelength: 32?,temporal frequency: 4 Hz, Michelsen contrast: 99.3%) intracellular freecalcium rised, leading to an increase in dye fluorescence (middle). Thetime course of relative fluorescence changes (_F/F
Basic Analysis of NimbleGen ChIP-on-chip Data using Bioconductor/R
Introduction Hybridization of chromatin immuno-precipitation (ChIP) material to tiling arrays at NimbleGen service facilities usually leaves the customer with a set of data files that are of limited use. Most information about the experiment
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