- ￥43274
- Beckman Coulter贝克曼库尔特
- 美国
- C31890
- 2025年07月12日
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文献和实验Two-hybrid analysis of genetic
in horizontal parallel stripes on a -u-h Glu plate.Streaks should be at least 3 mm wide and at least 5 mm apart,with the first streak starting about 15 mm from the edge of the plate.A 100 mm plate (which for some reason is typically 90 mm in diameter)will hold
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
of overnight culture to inoculate 500ml of LB (+antibiotic) x 2 for a total culture volume of 1L, shake overnight (about 16hrs) at 37°C. 3. Collect cells by centrifugation in 250ml x 4 Nalgene bottles, (H-6000A rotor, 3800 rpm), 10 minutes, 4°C.
Labeling Tubulin and Quantifying Labeling Stoichiometry
at 80K for 10' at 2¡C. 9. Recover the supernatant from the cold spin, add BRB80 to 1X (from a 5X stock), MgCl2 to 4 mM, GTP to 1 mM and incubate on ice for 3'. Warm to 37¡C for 2', add 1/2 volume of glycerol (33% v/v final), mix well and polymerize
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