Optic Filter Set, 355nm INDO，

Observation of living and plastic-embedded chick embryos

for several weeks before being set, producing embryos upon demand. Embedded embryos 1. Examine plastic embedded specimens of "13-hour" or "16-hour", "24-hour", "33-hour","48-hour" and "72-hour" chick embryos. These terms refer to classic studies of chick

Replication timing by density transfer

), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend

Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)

underneath the filter during the experiment. 3. Equilibrate the agonist dilutions to 37°C. D. Assay Set Up 1. The assay is performed with a 12 or 48 multi-well microchemotaxis chamber from Neuro Probe (cat. no. AP48). In addition to the steps described