- ￥47300
- Beckman Coulter贝克曼库尔特
- 美国
- A69750
- 2025年07月14日
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文献和实验Observation of living and plastic-embedded chick embryos
for several weeks before being set, producing embryos upon demand. Embedded embryos 1. Examine plastic embedded specimens of "13-hour" or "16-hour", "24-hour", "33-hour","48-hour" and "72-hour" chick embryos. These terms refer to classic studies of chick
Replication timing by density transfer
), arrest with 200 nM alpha factor (1-1.25 doubling times, until % unbudded cells stops increasing). NOTE: This alpha factor concentration is for bar1 strains. BAR1+ strains require 15x more alpha factor. 3. Filter, wash in Ycomp+alpha factor, resuspend
Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
underneath the filter during the experiment. 3. Equilibrate the agonist dilutions to 37°C. D. Assay Set Up 1. The assay is performed with a 12 or 48 multi-well microchemotaxis chamber from Neuro Probe (cat. no. AP48). In addition to the steps described
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