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文献和实验Real-Time qRT-PCR standard curves... efficiency is too h
Greetings, In my Real-Time qRT-PCR experiments, I employ the standard curve method for quantification of gene expression. However, standard curves seem to be a huge hit-or-miss procedure for me, even with genes that are well-established
To reach these goals, the necessary work was structured in six thematic fields (work packages): Standards for Proteomics Data Representation, Standards Implementation, Data Integration Tools, Proteomics Repository Adaptation, Data Flow Management
Functional Stem Cell Integration Assessed by Organotypic Slice Cultures
and the host tissue. Therefore, it is of fundamental interest to establish protocols that allow us to delineate a detailed time course of grafted stem cell survival, migration, differentiation, integration, and functional interaction with the host. One option
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