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文献和实验Procedures Common to the First Five Behavioral Tests
ceiling lights. There should be no unusual and distinctive odors or noises in the vicinity. Transport to testing room The experimenter will carry one or two (for open field) cages from the holding rack to the test room. First the water bottle
Transformation of E. coli by Electroporation
autoclaved bottles!). 4. Wash the cell pellet by resuspending in 250 ml of sterile ice-cold WB (10% redistilled glycerol, 90% distilled water, v/v). Centrifuge the cell suspension at 5,000 RPM for 15 min and carefully pour off the supernate as soon
by pairs into a new home cage (i.e., bedding change) with a water bottle that has a sipper tube (something that Portland site lacks on routinely used water bottles). They are then moved to the drinking study room (if this room differs from the colony
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