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文献和实验Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
Endurance Training Protocol and Longitudinal Performance Assays for Drosophila melanogaster
from Grainger rests on top of four 2"x6" boards and one ¾ inch plywood board. The motor, an AC/DC speed control, a fuse, and an on/off switch are wired together into a juncture box. 2) To cool the motor, a hole 3 inches in diameter is cut
Transformation of E. coli by Electroporation电穿孔转化E.coli
. 12.Turn power switches on for both the power booster and pulse control apparati. Voltage booster will display -16.66. 13.Charge pulse control by setting the CHARGE/ARM switch to CHARGE, then press and hold down the UP voltage control button
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