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文献和实验Microinjection DNA Purification
onto the cartridge. Spin at maximum speed for 10 seconds in a microcentrifuge. Reload the flowthrough and spin again. Discard the flowthrough after the second spin. The spin filter has a capacity of 20 micrograms DNA, so you can run several 700 microliters loads
RNA Isolation Protocol from LCM Samples
with 30 μl of Lysis Solution for >= 5 min a. Prewet the Micro Filter Cartridge Assembly by applying 30 μl of Lysis Solution to the center of the filter and allow it to soak while performing the next two steps. b. After completing steps
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
. Put the folded filter onto a 50 ml tube or equilibrated Nucleobond AX cartridge, wet it with 0.5 - 1.0 ml of water and fill it with the cooled lysate. Collect the cleared flowthrough. These filters are suitable for plasmid isolation with Nucleobond AX 100
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