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文献和实验Whole mount in situ hybridization with digoxygenin probes
Whole mount in situ hybridization with digoxygenin probes 1. Probe labelling (DNA) DNA template (100 ng - 1 µg ) in dH20 5 µl pd(N)6 (10 mg/ml) 10 µl Mix template and hexamer, boil 5 min, chill on ice 3 min then add
RNA Whole Mount In Situ Hybridization
with fresh, add RNA probe (typically anywhere from one-fiftieth to one-tenth of a transcription reaction) and incubate overnight at 70° C. Day 2: Post-hybridization washes, blocking, and antibody incubation Prewarm solution I to 70°C
We describe here a two-colour FISH method for interphase nuclei of whole mount drosophila embryos. This procedure has been adapted from the protocol described by Gemkow et al , 1998, and allows us to study the relative position of two distinct single copy genes in the nucleus
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