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文献和实验Small RNA Sequencing Sample Preparation Guide
Small RNA Sequencing Sample Preparation Guide.pdf
. The sequence of the 2-nt 3'' overhang makes a small contribution to the specificity of target recognition restricted to the unpaired nucleotide adjacent to the first base pair. 2''-Deoxynucleotides in the 3'' overhangs are as efficient as ribonucleotides
a complementary sequence of bases to any single strand of DNA providing it has a double stranded starting point. This is very useful because you can select which target gene you wish the polymerase to amplify in a mixed DNA sample by adding small pieces
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