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- Beckman Coulter贝克曼库尔特
- 美国
- 347267
- 2025年07月13日
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文献和实验Preparation of Embryonic Extract
. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml
Preparation of Embryonic Extract
. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml conical disposable centrifuge tubes
Sucrose Density Gradient Fractionation of Yeast Membranes
tubes with 30% sucrose solution (weigh them to confirm balance) Spin in swinging bucket centrifuge for 16 hr (can go longer) at 190,000 x g (37500 rpm) in a SW55Ti rotor Have microfuge tubes pre-labelled with 100 ul 4X SDS PAGE sample
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