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文献和实验Searching Haptens, Carrier Proteins, and Anti-Hapten Antibodies
Haptens are small molecules that are usually nonimmunogenic unless coupled to some carrier proteins. The generation of anti-hapten antibodies is important for the development of immunodiagnostics and therapeutics. Recently, our group
Procedure for fixing and staining adherent cells for immunofluorescence
serum is for blocking purposes and should correspond to species that secondary antibody is developed in. For example, if you are using goat anti-rabbit 488 and goat anti-mouse 568, you should use 5 normal goat serum for blocking 8. Place 100uL
XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂:PBS(磷酸盐缓冲液pH7.4)(实验室常规配置);非免疫山羊血清;Triton X-100(sigma);BSA抗体稀释液(实验室常规配置);A蛋白抗体(Santa cruz);B蛋白抗体(Abcam);Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen);Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
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