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文献和实验receptor type I is designed and validated. This experimental procedure was used to determine the abundance of growth hormone receptor type I transcript in different tilapia tissues. The results obtained with this developed competitive RT-PCR were similar
Staining Procedure for Flow Cytometric Detection of Human Cyclins
fetal bovine serum. The pH of the Wash buffer should be 7.1�7.4. REAGENTS: Wash buffer stored at 4°C, 75% ethanol stored at �20°C, Pure methanol stored at -20°C, 1% formaldehyde (methanol-free) in PBS, stored at 4°C, 0.25% Triton ® X-100 in Wash
to bring the pH down. After this adjustment, The color of the gel/Binding buffer mixture should be light yellow. 4) Apply 700 ul of the DNA/agarose solution to a HiBind® MicroEluteTM DNA column assembled in a clean 2 mL collection tube (provided
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